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Effects of Erythropoietin and GCSF on Traumatic Osteonecrosis of the Femoral Head in Rabbits

Mohammad Ghoreishian MD1*, Jalaledin Ghanavi MD 2, Mahsa Ahadi MD 2, Alisina Shahi MD3, Timothy L. Tan MD3, Hamid Reza Seyyed Hosseinzadeh MD1

1 Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 National Research Institute of Tuberculosis and Lung Disease, Tehran, Iran
3 Rothman Institute, Philadelphia, USA
* Corresponding Author

Introduction: To assess the effects of Erythropoietin and Granulocyte colony-stimulating factor on traumatic osteonecrosis of the femoral head in rabbits.

Materials and methods: 18 female rabbits were induced with traumatic osteonecrosis of the right femoral head by standardized procedure then were divided into three groups: (1) administered post-op 5000 IU/kg intraperitoneal erythropoietin, (2) administered post-op 5000 IU/kg intraperiotneal erythropoietin and daily SC injections of 300 ╬╝gr/kg of GCSF for 5 days, and (3) control group. The histologic comparisons of necrosis and empty lacunae of the three groups were compared at weeks 2 and 4.

Results: After 2 weeks in the EPO group showed 40% necrosis and 27% empty lacunae, whereas after 4 weeks, showed 10% necrosis and 10% empty lacunae(p0.017 and p=0.05, respectively). Also comparing the percent necrosis and empty lacunae after 4-weeks in the EPO group with the 2 week control group showed statistically significant reduction in both necrosis(10% vs. 50%) and empty lacunae(10% vs. 40%)(p=0.048).

Conclusion: In conclusion, the intraperitoneal administration of single dose EPO can effectively prevent the amount of necrosis in rabbit model of ONFH after 4 weeks.

Keywords: Erythropoietin, Granulocyte colony-stimulating factor, AVN, femoral head, Orthopaedics, A.6, A.9, A.10